MK886, a potent and specific leukotriene biosynthesis inhibitor blocks and reverses the membrane association of 5-lipoxygenase in ionophore-challenged leukocytes.

CA Rouzer, AW Ford-Hutchinson, HE Morton… - Journal of Biological …, 1990 - ASBMB
Recently, we have shown that ionophore activation of human leukocytes results in
leukotriene synthesis and a translocation of 5-lipoxygenase from the cytosol to cellular
membrane. This membrane translocation was postulated to be an important early activation
step for the enzyme. 3-[1-(p-Chlorobenzyl)-5-(isopropyl)-3-tert-butylthioindol-2-yl]-2, 2-
dimethylpropanoic acid (MK886) is a potent and specific inhibitor of leukotriene biosynthesis
in vivo and in intact cells, but has no direct effect on 5-lipoxygenase activity in cell-free …