Glucose-induced oscillations of cytoplasmic Ca2+ in the pancreatic β-cell
E Grapengiesser, E Gylfe, B Hellman - Biochemical and biophysical …, 1988 - Elsevier
E Grapengiesser, E Gylfe, B Hellman
Biochemical and biophysical research communications, 1988•ElsevierThe cytoplasmic calcium concentration (Ca 2+ i) was measured in individual mouse
pancreatic β-cells loaded with fura-2 by recording the 340/380 nm fluorescence excitation
ratio. An increase of the glucose concentration from 3 to 20 mM, caused initial lowering of
Ca 2+ i followed by a rise with a peak preceding constant elevation at an intermediary level.
However, at 11 mM glucose there were large Ca 2+ i oscillations with a frequency of 1 cycle
per 2–6 min. The results indicate that both first and second phase secretion depend on …
pancreatic β-cells loaded with fura-2 by recording the 340/380 nm fluorescence excitation
ratio. An increase of the glucose concentration from 3 to 20 mM, caused initial lowering of
Ca 2+ i followed by a rise with a peak preceding constant elevation at an intermediary level.
However, at 11 mM glucose there were large Ca 2+ i oscillations with a frequency of 1 cycle
per 2–6 min. The results indicate that both first and second phase secretion depend on …
The cytoplasmic calcium concentration (Ca2+i) was measured in individual mouse pancreatic β-cells loaded with fura-2 by recording the 340/380 nm fluorescence excitation ratio. An increase of the glucose concentration from 3 to 20 mM, caused initial lowering of Ca2+i followed by a rise with a peak preceding constant elevation at an intermediary level. However, at 11 mM glucose there were large Ca2+i oscillations with a frequency of 1 cycle per 2–6 min. The results indicate that both first and second phase secretion depend on elevated Ca2+i, and that many electrically coupled cells collectively determine the pace of rhythmic depolarization.
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