Identification of homozygous and heterozygous dy2J mice by PCR
The dystrophia muscularisdy2J/dy2J mouse is an animal model for one form of human
congenital muscular dystrophy. A point mutation in the gene coding for the laminin-2 α2
chain leads to the expression of a truncated, partially functional protein. We developed a
simple assay for the detection of the dy2J allele, which contains a new NdeI restriction site.
Genomic DNA was prepared from animals of known status and amplified by PCR. The
digestion of the PCR product with the restriction enzyme resulted in characteristic profiles …
congenital muscular dystrophy. A point mutation in the gene coding for the laminin-2 α2
chain leads to the expression of a truncated, partially functional protein. We developed a
simple assay for the detection of the dy2J allele, which contains a new NdeI restriction site.
Genomic DNA was prepared from animals of known status and amplified by PCR. The
digestion of the PCR product with the restriction enzyme resulted in characteristic profiles …