Purification of oxysterol binding protein from hamster liver cytosol

PA Dawson, DR Van der Westhuyzen… - Journal of Biological …, 1989 - ASBMB
We have purified to apparent homogeneity an oxysterol binding protein from cytosol of
hamster livers. This protein, which corresponds to the protein described by Taylor and
Kandutsch (Taylor, FR, and Kandutsch, A.(1985) Chem. Phys. Lipids 38, 187–194), binds
oxysterols such as 25-hydroxycholesterol but does not bind cholesterol or steroid hormones
in vitro. It may participate in the feedback repression of enzymes of cholesterol biosynthesis
and the low density lipoprotein receptor. The protein was purified more than 40,000-fold with …