[HTML][HTML] An Antibody Biosensor Establishes the Activation of the M1 Muscarinic Acetylcholine Receptor during Learning and Memory*♦
AJ Butcher, SJ Bradley, R Prihandoko… - Journal of Biological …, 2016 - ASBMB
Establishing the in vivo activation status of G protein-coupled receptors would not only
indicate physiological roles of G protein-coupled receptors but would also aid drug
discovery by establishing drug/receptor engagement. Here, we develop a phospho-specific
antibody-based biosensor to detect activation of the M 1 muscarinic acetylcholine receptor
(M 1 mAChR) in vitro and in vivo. Mass spectrometry phosphoproteomics identified 14 sites
of phosphorylation on the M 1 mAChR. Phospho-specific antibodies to four of these sites …
indicate physiological roles of G protein-coupled receptors but would also aid drug
discovery by establishing drug/receptor engagement. Here, we develop a phospho-specific
antibody-based biosensor to detect activation of the M 1 muscarinic acetylcholine receptor
(M 1 mAChR) in vitro and in vivo. Mass spectrometry phosphoproteomics identified 14 sites
of phosphorylation on the M 1 mAChR. Phospho-specific antibodies to four of these sites …