Simplified analysis of acetaminophen glucuronide for quantifying gluconeogenesis and glycogenolysis using deuterated water

J Jones, S Kahl, F Carvalho, C Barosa, M Roden - Analytical biochemistry, 2015 - Elsevier
J Jones, S Kahl, F Carvalho, C Barosa, M Roden
Analytical biochemistry, 2015Elsevier
Measurement of acetaminophen glucuronide (AG) 2 H enrichment from deuterated water (2
H 2 O) by 2 H nuclear magnetic resonance (NMR) analysis of its monoacetone glucose
(MAG) derivative provides estimation of gluconeogenic and glycogenolytic contributions to
endogenous glucose production (EGP). However, AG derivatization to MAG is laborious and
unsuitable for high-throughput studies. An alternative derivative, 5-O-acetyl monoacetone
glucuronolactone (MAGLA), was tested. Eleven healthy subjects ingested 2 H 2 O to 0.5 …
Abstract
Measurement of acetaminophen glucuronide (AG) 2H enrichment from deuterated water (2H2O) by 2H nuclear magnetic resonance (NMR) analysis of its monoacetone glucose (MAG) derivative provides estimation of gluconeogenic and glycogenolytic contributions to endogenous glucose production (EGP). However, AG derivatization to MAG is laborious and unsuitable for high-throughput studies. An alternative derivative, 5-O-acetyl monoacetone glucuronolactone (MAGLA), was tested. Eleven healthy subjects ingested 2H2O to 0.5% body water enrichment and 500 mg of acetaminophen. Plasma glucose and urinary glucuronide positional 2H enrichments were measured by 2H NMR spectroscopy of MAG and MAGLA, respectively. A Bland–Altman analysis indicated agreement at the 95% confidence level between glucose and glucuronide estimates.
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