A high-throughput approach for measuring temporal changes in the interactome
AR Kristensen, J Gsponer, LJ Foster - Nature methods, 2012 - nature.com
AR Kristensen, J Gsponer, LJ Foster
Nature methods, 2012•nature.comInteractomes are often measured using affinity purification–mass spectrometry (AP-MS) or
yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal
information. We combine quantitative proteomics and size-exclusion chromatography to
map 291 coeluting complexes. This method allows mapping of an interactome to the same
depth and accuracy as AP-MS with less work and without overexpression or tagging. The
use of triplex labeling enables monitoring of interactome rearrangements.
yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal
information. We combine quantitative proteomics and size-exclusion chromatography to
map 291 coeluting complexes. This method allows mapping of an interactome to the same
depth and accuracy as AP-MS with less work and without overexpression or tagging. The
use of triplex labeling enables monitoring of interactome rearrangements.
Abstract
Interactomes are often measured using affinity purification–mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use of triplex labeling enables monitoring of interactome rearrangements.
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