Control of insulin gene expression in pancreatic beta-cells and in an insulin-producing cell line, RIN-5F cells. II. Regulation of insulin mRNA stability.
M Welsh, DA Nielsen, AJ MacKrell… - Journal of Biological …, 1985 - ASBMB
The half-life of insulin mRNA at various glucose concentrations was determined by filter
hybridization techniques in isolated rat islets incubated with 3H-labeled uridine followed by
a chase incubation at 3.3 or 17 mM glucose. High glucose induced a greater stabilization of
insulin mRNA than of other poly (A)+ RNAs or total cellular RNA. In RIN-5F insulinoma cells,
an insulin-producing cell line, cholera toxin, but not glucose, induced a stabilization of
insulin mRNA. After 24 h of culture of islets with actinomycin D or alpha-amanitin at several …
hybridization techniques in isolated rat islets incubated with 3H-labeled uridine followed by
a chase incubation at 3.3 or 17 mM glucose. High glucose induced a greater stabilization of
insulin mRNA than of other poly (A)+ RNAs or total cellular RNA. In RIN-5F insulinoma cells,
an insulin-producing cell line, cholera toxin, but not glucose, induced a stabilization of
insulin mRNA. After 24 h of culture of islets with actinomycin D or alpha-amanitin at several …