Generation of Epstein‐Barr Virus (EBV)–Immortalized B Cell Lines
G Tosato, JI Cohen - Current protocols in immunology, 2007 - Wiley Online Library
G Tosato, JI Cohen
Current protocols in immunology, 2007•Wiley Online LibraryImmortalization of B lymphocytes by EBV is an effective procedure for inducing long‐term
growth of certain human B lymphocytes. The protocol described in this unit to accomplish
this can be divided into three stages: preparation of virus, preparation of target cells to be
immortalized, and EBV infection and growth of infected cells.
growth of certain human B lymphocytes. The protocol described in this unit to accomplish
this can be divided into three stages: preparation of virus, preparation of target cells to be
immortalized, and EBV infection and growth of infected cells.
Abstract
Immortalization of B lymphocytes by EBV is an effective procedure for inducing long‐term growth of certain human B lymphocytes. The protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infection and growth of infected cells.
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