Quantitative measurement of nuclear translocation events using similarity analysis of multispectral cellular images obtained in flow
TC George, SL Fanning, P Fitzgeral-Bocarsly… - Journal of …, 2006 - Elsevier
Nuclear translocation of NF-κB initiates transcription of numerous genes, many of which are
critical to host defense. Fluorescent image-based methods that quantify this event have
historically utilized adherent cells with large cytoplasm-to-nuclear area ratios. However,
many immunologically relevant cells are naturally non-adherent and have small cytoplasm-
to-nuclear area ratios. Using the ImageStream® imaging flow cytometer, we have developed
a novel method that measures nuclear translocation in large populations using cross …
critical to host defense. Fluorescent image-based methods that quantify this event have
historically utilized adherent cells with large cytoplasm-to-nuclear area ratios. However,
many immunologically relevant cells are naturally non-adherent and have small cytoplasm-
to-nuclear area ratios. Using the ImageStream® imaging flow cytometer, we have developed
a novel method that measures nuclear translocation in large populations using cross …