Aminoglycoside antibiotics traffic to the Golgi complex in LLC-PK1 cells.

R Sandoval, J Leiser, BA Molitoris - Journal of the American …, 1998 - journals.lww.com
Aminoglycoside antibiotics are known to be internalized via endocytosis and have been
associated with subcellular organelle dysfunction; however, the route of intracellular
trafficking and their distribution remain largely unknown. To address these questions, a
Texas Red conjugate of gentamicin (TRG) was synthesized for dual-labeling experiments
with the endoplasmic reticulum, Golgi, and lysosomal markers DiOC6-3, C6-NBD-ceramide,
and fluorescent dextrans, respectively. Confocal images were overlaid to determine areas of …