Cytosolic reducing power preserves glutamate in retina
J Du, W Cleghorn, L Contreras… - Proceedings of the …, 2013 - National Acad Sciences
J Du, W Cleghorn, L Contreras, JD Linton, GCK Chan, AO Chertov, T Saheki, V Govindaraju…
Proceedings of the National Academy of Sciences, 2013•National Acad SciencesGlutamate in neurons is an important excitatory neurotransmitter, but it also is a key
metabolite. We investigated how glutamate in a neural tissue is protected from catabolism.
Flux analysis using 13C-labeled fuels revealed that retinas use activities of the malate
aspartate shuttle to protect> 98% of their glutamate from oxidation in mitochondria. Isolation
of glutamate from the oxidative pathway relies on cytosolic NADH/NAD+, which is influenced
by extracellular glucose, lactate, and pyruvate.
metabolite. We investigated how glutamate in a neural tissue is protected from catabolism.
Flux analysis using 13C-labeled fuels revealed that retinas use activities of the malate
aspartate shuttle to protect> 98% of their glutamate from oxidation in mitochondria. Isolation
of glutamate from the oxidative pathway relies on cytosolic NADH/NAD+, which is influenced
by extracellular glucose, lactate, and pyruvate.
Glutamate in neurons is an important excitatory neurotransmitter, but it also is a key metabolite. We investigated how glutamate in a neural tissue is protected from catabolism. Flux analysis using 13C-labeled fuels revealed that retinas use activities of the malate aspartate shuttle to protect >98% of their glutamate from oxidation in mitochondria. Isolation of glutamate from the oxidative pathway relies on cytosolic NADH/NAD+, which is influenced by extracellular glucose, lactate, and pyruvate.
National Acad Sciences