[HTML][HTML] Modulation of subthalamic T-type Ca2+ channels remedies locomotor deficits in a rat model of Parkinson disease

CH Tai, YC Yang, MK Pan, CS Huang… - The Journal of clinical …, 2011 - Am Soc Clin Investig
CH Tai, YC Yang, MK Pan, CS Huang, CC Kuo
The Journal of clinical investigation, 2011Am Soc Clin Investig
An increase in neuronal burst activities in the subthalamic nucleus (STN) is a well-
documented electrophysiological feature of Parkinson disease (PD). However, the causal
relationship between subthalamic bursts and PD symptoms and the ionic mechanisms
underlying the bursts remain to be established. Here, we have shown that T-type Ca2+
channels are necessary for subthalamic burst firing and that pharmacological blockade of T-
type Ca2+ channels reduces motor deficits in a rat model of PD. Ni2+, mibefradil, NNC 55 …
An increase in neuronal burst activities in the subthalamic nucleus (STN) is a well-documented electrophysiological feature of Parkinson disease (PD). However, the causal relationship between subthalamic bursts and PD symptoms and the ionic mechanisms underlying the bursts remain to be established. Here, we have shown that T-type Ca2+ channels are necessary for subthalamic burst firing and that pharmacological blockade of T-type Ca2+ channels reduces motor deficits in a rat model of PD. Ni2+, mibefradil, NNC 55-0396, and efonidipine, which inhibited T-type Ca2+ currents in acutely dissociated STN neurons, but not Cd2+ and nifedipine, which preferentially inhibited L-type or the other non–T-type Ca2+ currents, effectively diminished burst activity in STN slices. Topical administration of inhibitors of T-type Ca2+ channels decreased in vivo STN burst activity and dramatically reduced the locomotor deficits in a rat model of PD. Cd2+ and nifedipine showed no such electrophysiological and behavioral effects. While low-frequency deep brain stimulation (DBS) has been considered ineffective in PD, we found that lengthening the duration of the low-frequency depolarizing pulse effectively improved behavioral measures of locomotion in the rat model of PD, presumably by decreasing the availability of T-type Ca2+ channels. We therefore conclude that modulation of subthalamic T-type Ca2+ currents and consequent burst discharges may provide new strategies for the treatment of PD.
The Journal of Clinical Investigation