Chloride transport in NCL-SG3 sweat gland cells: channels involved
Z Servetnyk, GM Roomans - Experimental and molecular pathology, 2007 - Elsevier
The aim of the study was to assess whether NCL-SG3, the only immortalized sweat gland
cell line available, can be used as an in vitro model to study chloride ion transport in cultured
sweat gland cells. Cl− efflux was measured using the MQAE dye fluorescence technique
after stimulating the cells with different agonists. A significant stimulation of chloride efflux
was achieved with the calcium ionophore A23187 resulting in an efflux rate of 0.9 mM/s.
Both ATP and UTP activated chloride efflux in these cells, with the ATP response being …
cell line available, can be used as an in vitro model to study chloride ion transport in cultured
sweat gland cells. Cl− efflux was measured using the MQAE dye fluorescence technique
after stimulating the cells with different agonists. A significant stimulation of chloride efflux
was achieved with the calcium ionophore A23187 resulting in an efflux rate of 0.9 mM/s.
Both ATP and UTP activated chloride efflux in these cells, with the ATP response being …