Calcium-activated chloride fluxes in cultured NCL-SG3 sweat gland cells
A Ring, AC Mörk, GM Roomans - Cell biology international, 1995 - Elsevier
The dependence of chloride permeability of the human sweat gland cell line NCL-SG3 cell
line on cytosolic free calcium ([Ca2+] i) was investigated. X-ray microanalysis, fura-2
fluorescence and patch clamp methodology were used. Carbachol and A23187 decreased
cellular Cl and K for cells grown on permeable supports, but carbachol had no effect on cells
grown on impermeable supports. In perforated patch experiments with impermeable
supports, ATP and calcium ionophores increased the inward current (ic) whereas carbachol …
line on cytosolic free calcium ([Ca2+] i) was investigated. X-ray microanalysis, fura-2
fluorescence and patch clamp methodology were used. Carbachol and A23187 decreased
cellular Cl and K for cells grown on permeable supports, but carbachol had no effect on cells
grown on impermeable supports. In perforated patch experiments with impermeable
supports, ATP and calcium ionophores increased the inward current (ic) whereas carbachol …