[HTML][HTML] Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets
R Shirakawa, T Higashi, A Tabuchi, A Yoshioka… - Journal of Biological …, 2004 - ASBMB
Platelets store self-agonists such as ADP and serotonin in dense core granules. Although
exocytosis of these granules is crucial for hemostasis and thrombosis, the underlying
mechanism is not fully understood. Here, we show that incubation of permeabilized platelets
with unprenylated active mutant Rab27A-Q78L, wild type Rab27A, and Rab27B inhibited
the secretion, whereas inactive mutant Rab27A-T23N and other GTPases had no effects.
Furthermore, we affinity-purified a GTP-Rab27A-binding protein in platelets and identified it …
exocytosis of these granules is crucial for hemostasis and thrombosis, the underlying
mechanism is not fully understood. Here, we show that incubation of permeabilized platelets
with unprenylated active mutant Rab27A-Q78L, wild type Rab27A, and Rab27B inhibited
the secretion, whereas inactive mutant Rab27A-T23N and other GTPases had no effects.
Furthermore, we affinity-purified a GTP-Rab27A-binding protein in platelets and identified it …