Determination of glucose-6-phosphatase activity using the glucose dehydrogenase-coupled reaction
M Alegre, CJ Ciudad, C Fillat, JJ Guinovart - Analytical biochemistry, 1988 - Elsevier
M Alegre, CJ Ciudad, C Fillat, JJ Guinovart
Analytical biochemistry, 1988•ElsevierWe present a method to determine glucose 6-phosphate activity. This assay measures the
rate of glucose released in the glucose-6-phosphatase reaction. The glucose is oxidized to β-
d-gluconolactone by glucose dehydrogenase in a coupled reaction that uses NAD (P)+. The
determination is rapid, reproducible, and does not require withdrawal, precipitation,
centrifugation, or neutralization steps. This method provides a simple resolution to the
problem of the nonspecific appearance of Pi, which is especially important in studies of …
rate of glucose released in the glucose-6-phosphatase reaction. The glucose is oxidized to β-
d-gluconolactone by glucose dehydrogenase in a coupled reaction that uses NAD (P)+. The
determination is rapid, reproducible, and does not require withdrawal, precipitation,
centrifugation, or neutralization steps. This method provides a simple resolution to the
problem of the nonspecific appearance of Pi, which is especially important in studies of …
We present a method to determine glucose 6-phosphate activity. This assay measures the rate of glucose released in the glucose-6-phosphatase reaction. The glucose is oxidized to β-d-gluconolactone by glucose dehydrogenase in a coupled reaction that uses NAD(P)+. The determination is rapid, reproducible, and does not require withdrawal, precipitation, centrifugation, or neutralization steps. This method provides a simple resolution to the problem of the nonspecific appearance of Pi, which is especially important in studies of regulation of glucose-6-phosphatase performed in the presence of ATP.
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