Reduction of macrophage infiltration and chemoattractant gene expression changes in white adipose tissue of morbidly obese subjects after surgery-induced weight …

R Cancello, C Henegar, N Viguerie, S Taleb… - Diabetes, 2005 - Am Diabetes Assoc
R Cancello, C Henegar, N Viguerie, S Taleb, C Poitou, C Rouault, M Coupaye, V Pelloux…
Diabetes, 2005Am Diabetes Assoc
In human obesity, the stroma vascular fraction (SVF) of white adipose tissue (WAT) is
enriched in macrophages. These cells may contribute to low-grade inflammation and to its
metabolic complications. Little is known about the effect of weight loss on macrophages and
genes involved in macrophage attraction. We examined subcutaneous WAT (scWAT) of 7
lean and 17 morbidly obese subjects before and 3 months after bypass surgery.
Immunomorphological changes of the number of scWAT-infiltrating macrophages were …
In human obesity, the stroma vascular fraction (SVF) of white adipose tissue (WAT) is enriched in macrophages. These cells may contribute to low-grade inflammation and to its metabolic complications. Little is known about the effect of weight loss on macrophages and genes involved in macrophage attraction. We examined subcutaneous WAT (scWAT) of 7 lean and 17 morbidly obese subjects before and 3 months after bypass surgery. Immunomorphological changes of the number of scWAT-infiltrating macrophages were evaluated, along with concomitant changes in expression of SVF-overexpressed genes. The number of scWAT-infiltrating macrophages before surgery was higher in obese than in lean subjects (HAM56+/CD68+; 22.6 ± 4.3 vs. 1.4 ± 0.6%, P < 0.001). Typical “crowns” of macrophages were observed around adipocytes. Drastic weight loss resulted in a significant decrease in macrophage number (−11.63 ± 2.3%, P < 0.001), and remaining macrophages stained positive for the anti-inflammatory protein interleukin 10. Genes involved in macrophage attraction (monocyte chemotactic protein [MCP]-1, plasminogen activator urokinase receptor [PLAUR], and colony-stimulating factor [CSF]-3) and hypoxia (hypoxia-inducible factor-1α [HIF-1α]), expression of which increases in obesity and decreases after surgery, were predominantly expressed in the SVF. We show that improvement of the inflammatory profile after weight loss is related to a reduced number of macrophages in scWAT. MCP-1, PLAUR, CSF-3, and HIF-1α may play roles in the attraction of macrophages in scWAT.
Am Diabetes Assoc