[PDF][PDF] IRF4 transcription-factor-dependent CD103+ CD11b+ dendritic cells drive mucosal T helper 17 cell differentiation

EK Persson, H Uronen-Hansson, M Semmrich… - Immunity, 2013 - cell.com
EK Persson, H Uronen-Hansson, M Semmrich, A Rivollier, K Hägerbrand, J Marsal
Immunity, 2013cell.com
Summary CD103+ CD11b+ dendritic cells (DCs) represent the major migratory DC
population within the small intestinal lamina propria (SI-LP), but their in vivo function
remains unclear. Here we demonstrate that intestinal CD103+ CD11b+ DC survival was
dependent on interferon regulatory factor 4 (IRF4). Mice with a DC deletion in Irf4 displayed
reduced numbers of intestinal interleukin 17 (IL-17)-secreting helper T 17 (Th17) cells and
failed to support Th17 cell differentiation in draining mesenteric lymph nodes (MLN) …
Summary
CD103+CD11b+ dendritic cells (DCs) represent the major migratory DC population within the small intestinal lamina propria (SI-LP), but their in vivo function remains unclear. Here we demonstrate that intestinal CD103+CD11b+ DC survival was dependent on interferon regulatory factor 4 (IRF4). Mice with a DC deletion in Irf4 displayed reduced numbers of intestinal interleukin 17 (IL-17)-secreting helper T 17 (Th17) cells and failed to support Th17 cell differentiation in draining mesenteric lymph nodes (MLN) following immunization. The latter was associated with a selective reduction in CD103+CD11b+ MLN DCs and DC derived IL-6. Immunized Il6−/− mice failed to support Th17 cell differentiation in MLN in vivo and CD103+CD11b+ MLN DCs supported IL-6-dependent Th17 cell differentiation in vitro. Together, our results suggest a central role for IRF4-dependent, IL-6 producing CD103+CD11b+ DCs in intestinal Th17 cell differentiation.
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