Isolation of methanobactin from the spent media of methane-oxidizing bacteria
NL Bandow, WH Gallagher, L Behling, DW Choi… - Methods in …, 2011 - Elsevier
Chalkophores are low molecular mass modified peptides involved in copper acquisition in
methane-oxidizing bacteria (MOB). A screening method for the detection of this copper-
binding molecule is presented in Chapter 16. Here we describe methods to (1) maximize
expression and secretion of chalkophores,(2) concentrate chalkophores from the spent
media of MOB, and (3) purify chalkophores.
methane-oxidizing bacteria (MOB). A screening method for the detection of this copper-
binding molecule is presented in Chapter 16. Here we describe methods to (1) maximize
expression and secretion of chalkophores,(2) concentrate chalkophores from the spent
media of MOB, and (3) purify chalkophores.