[HTML][HTML] Sildenafil stimulates human trophoblast invasion through nitric oxide and guanosine 3′, 5′-cyclic monophosphate signaling

JM Bolnick, BA Kilburn, AD Bolnick, MP Diamond… - Fertility and sterility, 2015 - Elsevier
Objective To determine the effect of sildenafil, a phosphodiesterase type 5 inhibitor, on
trophoblast invasiveness. Design Laboratory investigation. Setting Academic medical
center. Patient (s) Placental tissues discarded after first-trimester terminations were obtained
from patients with informed consent. Intervention (s) A cell line, HTR-8/SVneo, established
from first-trimester cytotrophoblast, and villous explants, was treated with or without
sildenafil, guanosine 3′, 5′-cyclic monophosphate (cGMP) analog, cGMP inhibitor, or L …