[HTML][HTML] Inflammation-induced endothelial cell-derived extracellular vesicles modulate the cellular status of pericytes

S Yamamoto, S Niida, E Azuma, T Yanagibashi… - Scientific reports, 2015 - nature.com
S Yamamoto, S Niida, E Azuma, T Yanagibashi, M Muramatsu, TT Huang, H Sagara…
Scientific reports, 2015nature.com
Emerging lines of evidence have shown that extracellular vesicles (EVs) mediate cell-to-cell
communication by exporting encapsulated materials, such as microRNAs (miRNAs), to
target cells. Endothelial cell-derived EVs (E-EVs) are upregulated in circulating blood in
different pathological conditions; however, the characteristics and the role of these E-EVs
are not yet well understood. In vitro studies were conducted to determine the role of
inflammation-induced E-EVs in the cell-to-cell communication between vascular endothelial …
Abstract
Emerging lines of evidence have shown that extracellular vesicles (EVs) mediate cell-to-cell communication by exporting encapsulated materials, such as microRNAs (miRNAs), to target cells. Endothelial cell-derived EVs (E-EVs) are upregulated in circulating blood in different pathological conditions; however, the characteristics and the role of these E-EVs are not yet well understood. In vitro studies were conducted to determine the role of inflammation-induced E-EVs in the cell-to-cell communication between vascular endothelial cells and pericytes/vSMCs. Stimulation with inflammatory cytokines and endotoxin immediately induced release of shedding type E-EVs from the vascular endothelial cells and flow cytometry showed that the induction was dose dependent. MiRNA array analyses revealed that group of miRNAs were specifically increased in the inflammation-induced E-EVs. E-EVs added to the culture media of cerebrovascular pericytes were incorporated into the cells. The E-EV-supplemented cells showed highly induced mRNA and protein expression of VEGF-B, which was assumed to be a downstream target of the miRNA that was increased within the E-EVs after inflammatory stimulation. The results suggest that E-EVs mediate inflammation-induced endothelial cell-pericyte/vSMC communication and the miRNAs encapsulated within the E-EVs may play a role in regulating target cell function. E-EVs may be new therapeutic targets for the treatment of inflammatory diseases.
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