Exosomes as a tumor vaccine: enhancing potency through direct loading of antigenic peptides
DH Hsu, P Paz, G Villaflor, A Rivas… - Journal of …, 2003 - journals.lww.com
Exosomes secreted by dendritic cells (DCs) contain MHC-I, MHC-II, and other accessory
molecules required for antigen presentation to T cells. Previous studies have shown that
exosome MHC-I “indirectly” loaded by adding peptides to DC cultures are immunogenic.
However, analysis of peptide binding was not performed to link T-cell-stimulating activity
with the amount of MHC-I/peptide complexes on the exosomes. In this study, we measured
peptide binding to MHC-I under different loading conditions and tested the exosomes' …
molecules required for antigen presentation to T cells. Previous studies have shown that
exosome MHC-I “indirectly” loaded by adding peptides to DC cultures are immunogenic.
However, analysis of peptide binding was not performed to link T-cell-stimulating activity
with the amount of MHC-I/peptide complexes on the exosomes. In this study, we measured
peptide binding to MHC-I under different loading conditions and tested the exosomes' …