Generation of HIV latency in humanized BLT mice

PW Denton, R Olesen, SK Choudhary… - Journal of …, 2012 - Am Soc Microbiol
PW Denton, R Olesen, SK Choudhary, NM Archin, A Wahl, MD Swanson, M Chateau…
Journal of virology, 2012Am Soc Microbiol
Here we demonstrate that a combination of tenofovir, emtricitabine, and raltegravir
effectively suppresses peripheral and systemic HIV replication in humanized BLT mice. We
also demonstrate that antiretroviral therapy (ART)-treated humanized BLT mice harbor
latently infected resting human CD4+ T cells that can be induced ex vivo to produce HIV. We
observed that the levels of infected resting human CD4+ T cells present in BLT mice are
within the range of those observed circulating in patients undergoing suppressive ART …
Abstract
Here we demonstrate that a combination of tenofovir, emtricitabine, and raltegravir effectively suppresses peripheral and systemic HIV replication in humanized BLT mice. We also demonstrate that antiretroviral therapy (ART)-treated humanized BLT mice harbor latently infected resting human CD4+ T cells that can be induced ex vivo to produce HIV. We observed that the levels of infected resting human CD4+ T cells present in BLT mice are within the range of those observed circulating in patients undergoing suppressive ART. These results demonstrate the potential of humanized BLT mice as an attractive model for testing the in vivo efficacy of novel HIV eradication strategies.
American Society for Microbiology