TNF-α-induced increase in intestinal epithelial tight junction permeability requires NF-κB activation

TY Ma, GK Iwamoto, NT Hoa, V Akotia… - American Journal …, 2004 - journals.physiology.org
TY Ma, GK Iwamoto, NT Hoa, V Akotia, A Pedram, MA Boivin, HM Said
American Journal of Physiology-Gastrointestinal and Liver …, 2004journals.physiology.org
Crohn's disease (CD) patients have an abnormal increase in intestinal epithelial
permeability. The defect in intestinal tight junction (TJ) barrier has been proposed as an
important etiologic factor of CD. TNF-α increases intestinal TJ permeability. Because TNF-α
levels are markedly increased in CD, TNF-α increase in intestinal TJ permeability could be a
contributing factor of intestinal permeability defect in CD. Our purpose was to determine
some of the intracellular mechanisms involved in TNF-α modulation of intestinal epithelial TJ …
Crohn's disease (CD) patients have an abnormal increase in intestinal epithelial permeability. The defect in intestinal tight junction (TJ) barrier has been proposed as an important etiologic factor of CD. TNF-α increases intestinal TJ permeability. Because TNF-α levels are markedly increased in CD, TNF-α increase in intestinal TJ permeability could be a contributing factor of intestinal permeability defect in CD. Our purpose was to determine some of the intracellular mechanisms involved in TNF-α modulation of intestinal epithelial TJ permeability by using an in vitro intestinal epithelial system consisting of filter-grown Caco-2 monolayers. TNF-α produced a concentration- and time-dependent increase in Caco-2 TJ permeability. TNF-α-induced increase in Caco-2 TJ permeability correlated with Caco-2 NF-κB activation. Inhibition of TNF-α-induced NF-κB activation by selected NF-κB inhibitors, curcumin and triptolide, prevented the increase in Caco-2 TJ permeability, indicating that NF-κB activation was required for the TNF-α-induced increase in Caco-2 TJ permeability. This increase in Caco-2 TJ permeability was accompanied by down-regulation of zonula occludens (ZO)-1 proteins and alteration in junctional localization of ZO-1 proteins. TNF-α modulation of ZO-1 protein expression and junctional localization were also prevented by NF-κB inhibitors. TNF-α did not induce apoptosis in Caco-2 cells, suggesting that apoptosis was not the mechanism involved in TNF-α-induced increase in Caco-2 TJ permeability. These results demonstrate for the first time that TNF-α-induced increase in Caco-2 TJ permeability was mediated by NF-κB activation. The increase in permeability was associated with NF-κB-dependent downregulation of ZO-1 protein expression and alteration in junctional localization.
American Physiological Society