Isolation of IgG antibody Fv-DNA from various mouse and rat hybridoma cell lines using the polymerase chain reaction with a simple set of primers
S Dübel, F Breitling, P Fuchs, M Zewe, S Gotter… - Journal of …, 1994 - Elsevier
To facilitate the isolation of IgG antibody Fv-DNA sequences from hybridoma cell lines, we
have established a polymerase chain reaction (PCR) procedure requiring only a small
number of primers. The sense primers homologous to DNA coding for the first framework
sequences were designed to hybridize to all the known antibody sequences under
conditions that permit a high number of mismatches. The antisense primers were
homologous to DNA coding for the beginning of the constant regions of the γ and κ chains …
have established a polymerase chain reaction (PCR) procedure requiring only a small
number of primers. The sense primers homologous to DNA coding for the first framework
sequences were designed to hybridize to all the known antibody sequences under
conditions that permit a high number of mismatches. The antisense primers were
homologous to DNA coding for the beginning of the constant regions of the γ and κ chains …