[PDF][PDF] The regulation of Notch signaling controls satellite cell activation and cell fate determination in postnatal myogenesis

IM Conboy, TA Rando - Developmental cell, 2002 - cell.com
Developmental cell, 2002cell.com
We have studied the role of Notch-1 and its antagonist Numb in the activation of satellite
cells during postnatal myogenesis. Activation of Notch-1 promoted the proliferation of
myogenic precursor cells expressing the premyoblast marker Pax3. Attenuation of Notch
signaling by increases in Numb expression led to the commitment of progenitor cells to the
myoblast cell fate and the expression of myogenic regulatory factors, desmin, and Pax7. In
many intermediate progenitor cells, Numb was localized asymmetrically in actively dividing …
Abstract
We have studied the role of Notch-1 and its antagonist Numb in the activation of satellite cells during postnatal myogenesis. Activation of Notch-1 promoted the proliferation of myogenic precursor cells expressing the premyoblast marker Pax3. Attenuation of Notch signaling by increases in Numb expression led to the commitment of progenitor cells to the myoblast cell fate and the expression of myogenic regulatory factors, desmin, and Pax7. In many intermediate progenitor cells, Numb was localized asymmetrically in actively dividing cells, suggesting an asymmetric cell division and divergent cell fates of daughter cells. The results indicate that satellite cell activation results in a heterogeneous population of precursor cells with respect to Notch-1 activity and that the balance between Notch-1 and Numb controls cellular homeostasis and cell fate determination.
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