Real-time fluorescence-based PCR for detection of malaria parasites

MA Lee, CH Tan, LT Aw, CS Tang… - Journal of clinical …, 2002 - Am Soc Microbiol
MA Lee, CH Tan, LT Aw, CS Tang, M Singh, SH Lee, HP Chia, EPH Yap
Journal of clinical microbiology, 2002Am Soc Microbiol
ABSTRACT A fluorescence-based real-time 5′ nuclease PCR capable of detecting all four
human malaria parasites was developed to screen large numbers of samples during an
outbreak to prevent further transmission of malaria. The effectiveness of antimalarial therapy
for malaria patients can be monitored by determining the reduction of parasitemia by this
method.
Abstract
A fluorescence-based real-time 5′ nuclease PCR capable of detecting all four human malaria parasites was developed to screen large numbers of samples during an outbreak to prevent further transmission of malaria. The effectiveness of antimalarial therapy for malaria patients can be monitored by determining the reduction of parasitemia by this method.
American Society for Microbiology