CD8⁺ T regulatory cells (T regs) are elicited by unique antigen presenting cells during viral infections, by manipulation of co-stimulatory molecules, or in the development of tumors. CD8⁺ T regs display antigen-specificity, which is most exquisitely manifested by the HLA-E-restricted cytolytic CD8⁺ T regs in MS. There is evidence that some CD8⁺ T regs also express organ specificity. In many cases, IFN-γ is required for either the induction or expression of CD8⁺ T regs. CD8⁺ T regs can produce suppression directly by killing immune cells or indirectly by co-opting other cells to elaborate end-stage suppressive molecules such as TGF-β, IL-10, and indoleamine dioxygenase (IDO).