Large-scale clinical-grade retroviral vector production in a fixed-bed bioreactor

X Wang, M Olszewska, J Qu… - Journal of …, 2015 - journals.lww.com
The successful genetic engineering of patient T cells with γ-retroviral vectors expressing
chimeric antigen receptors or T-cell receptors for phase II clinical trials and beyond requires
the large-scale manufacture of high-titer vector stocks. The production of retroviral vectors
from stable packaging cell lines using roller bottles or 10-to 40-layer cell factories is limited
by a narrow harvest window, labor intensity, open-system operations, and the requirement
for significant incubator space. To circumvent these shortcomings, we optimized the …