Development of an in vitro potency assay for therapeutic TGFβ antagonists: The A549 cell bioassay
ML Rapoza, D Fu, RA Sendak - Journal of immunological methods, 2006 - Elsevier
A bioassay was developed to assess the potency of TGFβ antagonists by measuring IL-11
production in TGFβ-1 treated human lung epithelial cells (A549). The production of IL-11 by
A549 cells, measured by ELISA, was shown to be proportional to the TGFβ-1 concentration.
The A549 cells were responsive to all three isoforms of TGFβ in the range of 3.0 ng/mL to 1.4
pg/mL, with an 18 to 24 h exposure time found to be within the linear portion of the bioassay
response range. The Effective Dose at 80% of the maximal response (ED80) of TGFβ-1 …
production in TGFβ-1 treated human lung epithelial cells (A549). The production of IL-11 by
A549 cells, measured by ELISA, was shown to be proportional to the TGFβ-1 concentration.
The A549 cells were responsive to all three isoforms of TGFβ in the range of 3.0 ng/mL to 1.4
pg/mL, with an 18 to 24 h exposure time found to be within the linear portion of the bioassay
response range. The Effective Dose at 80% of the maximal response (ED80) of TGFβ-1 …