Constitutively active mutant D816VKit induces megakayocyte and mast cell differentiation of early haemopoietic cells from murine foetal liver

PT Ferrao, TJ Gonda, LK Ashman - Leukemia research, 2003 - Elsevier
Mutations of Kit at position D816 have been implicated in mastocytosis, acute myeloid
leukaemia and germ cell tumours. Expression of this mutant Kit in cell lines results in factor-
independent growth, differentiation and increased survival in vitro and tumourigenicity in
vivo. Mutant D816VKit and wild-type Kit were expressed in murine primary haemopoietic
cells and grown in stem cell factor (SCF) or the absence of factors. Expression of D816VKit
did not lead to transformation as assessed by a colony assay, but resulted in enhanced …