Expression of Wilms' tumor suppressor in the liver with cirrhosis: relation to hepatocyte nuclear factor 4 and hepatocellular function
C Berasain, JI Herrero, ER Garcıa-Trevijano, MA Avila… - Hepatology, 2003 - Elsevier
The Wilms' tumor suppressor WT1 is a transcriptional regulator present in the fetal but not in
the mature liver. Its expression and functional role in liver diseases remains unexplored. In
this study, we analyzed WT1 expression by reverse-transcription polymerase chain reaction
(RT-PCR) and by immunohistochemistry in normal and diseased livers. In addition, we
performed in vitro studies in isolated rat hepatocytes to investigate WT1 regulation and
function. We detected WT1 messenger RNA (mRNA) in 18% of normal livers, 17% of chronic …
the mature liver. Its expression and functional role in liver diseases remains unexplored. In
this study, we analyzed WT1 expression by reverse-transcription polymerase chain reaction
(RT-PCR) and by immunohistochemistry in normal and diseased livers. In addition, we
performed in vitro studies in isolated rat hepatocytes to investigate WT1 regulation and
function. We detected WT1 messenger RNA (mRNA) in 18% of normal livers, 17% of chronic …