[HTML][HTML] Cells isolated from human periapical cysts express mesenchymal stem cell-like properties

M Marrelli, F Paduano, M Tatullo - International journal of biological …, 2013 - ncbi.nlm.nih.gov
M Marrelli, F Paduano, M Tatullo
International journal of biological sciences, 2013ncbi.nlm.nih.gov
We provide a detailed description of mesenchymal stem cells (MSCs) isolated from human
periapical cysts, which we have termed hPCy-MSCs. These cells have a fibroblast-like
shape and adhere to tissue culture plastic surfaces. hPCy-MSCs possess high proliferative
potential and self-renewal capacity properties. We characterised the immunophenotype of
hPCy-MSCs (CD73+, CD90+, CD105+, CD13+, CD29+, CD44+, CD45-, STRO-1+, CD146+)
by flow cytometry and immunofluorescence. hPCy-MSCs possess the potential to …
Abstract
We provide a detailed description of mesenchymal stem cells (MSCs) isolated from human periapical cysts, which we have termed hPCy-MSCs. These cells have a fibroblast-like shape and adhere to tissue culture plastic surfaces. hPCy-MSCs possess high proliferative potential and self-renewal capacity properties. We characterised the immunophenotype of hPCy-MSCs (CD73+, CD90+, CD105+, CD13+, CD29+, CD44+, CD45-, STRO-1+, CD146+) by flow cytometry and immunofluorescence. hPCy-MSCs possess the potential to differentiate into osteoblast-and adipocyte-like cells in vitro. Multi-potentiality was evaluated with culture-specific staining and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis for osteo/odontogenic and adipogenic markers. This is the first report to indicate that human periapical cysts contain cells with MSC-like properties. Taken together, our findings indicate that human periapical cysts could be a rich source of MSCs.
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