Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1

HJ Jang, Z Kokrashvili… - Proceedings of the …, 2007 - National Acad Sciences
HJ Jang, Z Kokrashvili, MJ Theodorakis, OD Carlson, BJ Kim, J Zhou, HH Kim, X Xu
Proceedings of the National Academy of Sciences, 2007National Acad Sciences
Glucagon-like peptide-1 (GLP-1), released from gut endocrine L cells in response to
glucose, regulates appetite, insulin secretion, and gut motility. How glucose given orally, but
not systemically, induces GLP-1 secretion is unknown. We show that human duodenal L
cells express sweet taste receptors, the taste G protein gustducin, and several other taste
transduction elements. Mouse intestinal L cells also express α-gustducin. Ingestion of
glucose by α-gustducin null mice revealed deficiencies in secretion of GLP-1 and the …
Glucagon-like peptide-1 (GLP-1), released from gut endocrine L cells in response to glucose, regulates appetite, insulin secretion, and gut motility. How glucose given orally, but not systemically, induces GLP-1 secretion is unknown. We show that human duodenal L cells express sweet taste receptors, the taste G protein gustducin, and several other taste transduction elements. Mouse intestinal L cells also express α-gustducin. Ingestion of glucose by α-gustducin null mice revealed deficiencies in secretion of GLP-1 and the regulation of plasma insulin and glucose. Isolated small bowel and intestinal villi from α-gustducin null mice showed markedly defective GLP-1 secretion in response to glucose. The human L cell line NCI-H716 expresses α-gustducin, taste receptors, and several other taste signaling elements. GLP-1 release from NCI-H716 cells was promoted by sugars and the noncaloric sweetener sucralose, and blocked by the sweet receptor antagonist lactisole or siRNA for α-gustducin. We conclude that L cells of the gut “taste” glucose through the same mechanisms used by taste cells of the tongue. Modulating GLP-1 secretion in gut “taste cells” may provide an important treatment for obesity, diabetes and abnormal gut motility.
National Acad Sciences