Activation of autophagy by inflammatory signals limits IL-1β production by targeting ubiquitinated inflammasomes for destruction

CS Shi, K Shenderov, NN Huang, J Kabat… - Nature …, 2012 - nature.com
CS Shi, K Shenderov, NN Huang, J Kabat, M Abu-Asab, KA Fitzgerald, A Sher, JH Kehrl
Nature immunology, 2012nature.com
Autophagosomes delivers cytoplasmic constituents to lysosomes for degradation, whereas
inflammasomes are molecular platforms activated by infection or stress that regulate the
activity of caspase-1 and the maturation of interleukin 1β (IL-1β) and IL-18. Here we show
that the induction of AIM2 or NLRP3 inflammasomes in macrophages triggered activation of
the G protein RalB and autophagosome formation. The induction of autophagy did not
depend on the adaptor ASC or capase-1 but was dependent on the presence of the …
Abstract
Autophagosomes delivers cytoplasmic constituents to lysosomes for degradation, whereas inflammasomes are molecular platforms activated by infection or stress that regulate the activity of caspase-1 and the maturation of interleukin 1β (IL-1β) and IL-18. Here we show that the induction of AIM2 or NLRP3 inflammasomes in macrophages triggered activation of the G protein RalB and autophagosome formation. The induction of autophagy did not depend on the adaptor ASC or capase-1 but was dependent on the presence of the inflammasome sensor. Blocking autophagy potentiated inflammasome activity, whereas stimulating autophagy limited it. Assembled inflammasomes underwent ubiquitination and recruited the autophagic adaptor p62, which assisted their delivery to autophagosomes. Our data indicate that autophagy accompanies inflammasome activation to temper inflammation by eliminating active inflammasomes.
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