[HTML][HTML] pH-dependent intraluminal organization of mucin granules in live human mucous/goblet cells

J Perez-Vilar, JC Olsen, M Chua… - Journal of Biological …, 2005 - ASBMB
To study the mechanism of gel-forming mucin packaging within mucin granules, we
generated human mucous/goblet cells stably expressing a recombinant MUC5AC domain
fused to green fluorescent protein (GFP). The fusion protein, named SHGFP-MUC5AC/CK,
accumulated in the granules together with native MUC5AC. Inhibition of protein synthesis or
disorganization of the Golgi complex did not result in diminished intragranular SHGFP-
MUC5AC/CK signals, consistent with long-term storage of the fusion protein. However …