Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor.

R Calligaris, S Bottardi, S Cogoi… - Proceedings of the …, 1995 - National Acad Sciences
R Calligaris, S Bottardi, S Cogoi, I Apezteguia, C Santoro
Proceedings of the National Academy of Sciences, 1995National Acad Sciences
GATA-1 is a zinc-finger transcription factor that plays a critical role in the normal
development of hematopoietic cell lineages. In human and murine erythroid cells a
previously undescribed 40-kDa protein is detected with GATA-1-specific antibodies. We
show that the 40-kDa GATA-1 (GATA-1s) is produced by the use of an internal AUG initiation
codon in the GATA-1 transcript. The GATA-1 proteins share identical binding activity and
form heterodimers in erythroleukemic cells but differ in their transactivation potential and in …
GATA-1 is a zinc-finger transcription factor that plays a critical role in the normal development of hematopoietic cell lineages. In human and murine erythroid cells a previously undescribed 40-kDa protein is detected with GATA-1-specific antibodies. We show that the 40-kDa GATA-1 (GATA-1s) is produced by the use of an internal AUG initiation codon in the GATA-1 transcript. The GATA-1 proteins share identical binding activity and form heterodimers in erythroleukemic cells but differ in their transactivation potential and in their expression in developing mouse embryos.
National Acad Sciences