Mouse bladder wall injection

CL Fu, CA Apelo, B Torres, KH Thai… - JoVE (Journal of Visualized …, 2011 - jove.com
Mouse bladder wall injection is a useful technique to orthotopically study bladder
phenomena, including stem cell, smooth muscle, and cancer biology. Before starting
injections, the surgical area must be cleaned with soap and water and antiseptic solution.
Surgical equipment must be sterilized before use and between each animal. Each mouse is
placed under inhaled isoflurane anesthesia (2-5% for induction, 1-3% for maintenance) and
its bladder exposed by making a midline abdominal incision with scissors. If the bladder is …