Isolation of single atrial and ventricular cells from the human heart.
JO Bustamante, T Watanabe, DA Murphy… - Canadian Medical …, 1982 - ncbi.nlm.nih.gov
JO Bustamante, T Watanabe, DA Murphy, TF McDonald
Canadian Medical Association Journal, 1982•ncbi.nlm.nih.govThe single isolated heart cell has recently emerged as a model for the study of the structure
and function of cardiac cells. Heart muscle cells of adult animals of various species have
been successfully isolated by enzymatic digestion of intact cardiac tissue. In this paper a
dissociation method that yields living cells from atrial and ventricular tissue of young and
adult humans is detailed. The cells retain the morphologic features of cells in intact cardiac
tissue, and they generate action potentials and contractions in response to electrical …
and function of cardiac cells. Heart muscle cells of adult animals of various species have
been successfully isolated by enzymatic digestion of intact cardiac tissue. In this paper a
dissociation method that yields living cells from atrial and ventricular tissue of young and
adult humans is detailed. The cells retain the morphologic features of cells in intact cardiac
tissue, and they generate action potentials and contractions in response to electrical …
Abstract
The single isolated heart cell has recently emerged as a model for the study of the structure and function of cardiac cells. Heart muscle cells of adult animals of various species have been successfully isolated by enzymatic digestion of intact cardiac tissue. In this paper a dissociation method that yields living cells from atrial and ventricular tissue of young and adult humans is detailed. The cells retain the morphologic features of cells in intact cardiac tissue, and they generate action potentials and contractions in response to electrical stimulation. The study of isolated human heart cells should make a valuable contribution to knowledge of the normal and diseased heart.
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