[HTML][HTML] PEP-19, an intrinsically disordered regulator of calmodulin signaling
QK Kleerekoper, JA Putkey - Journal of biological chemistry, 2009 - ASBMB
PEP-19 is a small calmodulin (CaM)-binding protein that greatly increases the rates of
association and dissociation of Ca 2+ from the C-domain of CaM, an effect that is mediated
by an acidic/IQ sequence in PEP-19. We show here using NMR that PEP-19 is an
intrinsically disordered protein, but with residual structure localized to its acidic/IQ motif. We
also show that the k on and k off rates for binding PEP-19 to apo-CaM are at least 50-fold
slower than for binding to Ca 2+-CaM. These data indicate that intrinsic disorder confers …
association and dissociation of Ca 2+ from the C-domain of CaM, an effect that is mediated
by an acidic/IQ sequence in PEP-19. We show here using NMR that PEP-19 is an
intrinsically disordered protein, but with residual structure localized to its acidic/IQ motif. We
also show that the k on and k off rates for binding PEP-19 to apo-CaM are at least 50-fold
slower than for binding to Ca 2+-CaM. These data indicate that intrinsic disorder confers …