Induction of human immunodeficiency virus type 1 (HIV-1)-specific cytolytic T lymphocyte responses in seronegative adults by a nonreplicating, host-range-restricted …

MA Egan, WA Pavlat, J Tartaglia… - Journal of Infectious …, 1995 - academic.oup.com
MA Egan, WA Pavlat, J Tartaglia, E Paoletti, KJ Weinhold, ML Clements, RF Siliciano
Journal of Infectious Diseases, 1995academic.oup.com
Abstract CD8+ cytolytic T lymphocytes (CTL) are likely to be an important component of
effective vaccines against human immunodeficiency virus type 1 (HIV-1). CTL can be
induced most effectively with live virus vectors. However, because of concerns about the
safety of such vectors, a nonreplicating canarypox vector (ALVAC) capable of expressing
foreign genes in mammalian cells has been developed. This study evaluated the capacity of
an ALVAC vector expressing the HIV-1MN envelope (env) glycoprotein to induce HIV-1 …
Abstract
CD8+ cytolytic T lymphocytes (CTL) are likely to be an important component of effective vaccines against human immunodeficiency virus type 1 (HIV-1). CTL can be induced most effectively with live virus vectors. However, because of concerns about the safety of such vectors, a nonreplicating canarypox vector (ALVAC) capable of expressing foreign genes in mammalian cells has been developed. This study evaluated the capacity of an ALVAC vector expressing the HIV-1MN envelope (env) glycoprotein to induce HIV-1-specific CTL in seronegative volunteers. Protocols were designed to determine whether immunization with ALVAC alone or in combination with subunit boosting could induce CTL in vaccinia-immune and -naive volunteers. A simple method for antigen-specific in vitro stimulation was used to detect CTL responses in HIV-1-seronegative vaccine recipients. The results indicate that low doses of a nonreplicating virus vector alone can elicit both CD4+ and CD8+ HIV-1-specific CTL in a subset of seronegative volunteers.
Oxford University Press