[HTML][HTML] The R-SNARE motif of tomosyn forms SNARE core complexes with syntaxin 1 and SNAP-25 and down-regulates exocytosis
K Hatsuzawa, T Lang, D Fasshauer, D Bruns… - Journal of Biological …, 2003 - ASBMB
Tomosyn is a 130-kDa syntaxin-binding protein that contains a large N-terminal domain with
WD40 repeats and a C-terminal domain homologous to R-SNAREs. Here we show that
tomosyn forms genuine SNARE core complexes with the SNAREs syntaxin 1 and SNAP-25.
In vitro studies with recombinant proteins revealed that complex formation proceeds from
unstructured monomers to a stable four-helical bundle. The assembled complex displayed
features typical for SNARE core complexes, including a profound hysteresis upon unfolding …
WD40 repeats and a C-terminal domain homologous to R-SNAREs. Here we show that
tomosyn forms genuine SNARE core complexes with the SNAREs syntaxin 1 and SNAP-25.
In vitro studies with recombinant proteins revealed that complex formation proceeds from
unstructured monomers to a stable four-helical bundle. The assembled complex displayed
features typical for SNARE core complexes, including a profound hysteresis upon unfolding …