[HTML][HTML] Gene transfection of H25A mutant heme oxygenase-1 protects cells against hydroperoxide-induced cytotoxicity
R Hori, M Kashiba, T Toma, A Yachie, N Goda… - Journal of Biological …, 2002 - ASBMB
Heme oxygenase (HO)-1 is a stress-inducible enzyme protecting cells against oxidative
stress, and mechanisms have been considered to depend exclusively on its enzyme activity.
This study aimed to examine if the protein lacking catalytic activities could also display such
resistance against oxidative stress. Stable transfectants of rat wild type HO-1 cDNA (HO-1-
U937) and those of its H25A mutant gene (mHO-1-U937) were established using human
monoblastic lymphoma cell U937. HO-1-U937 and mHO-1-U937 used in the study exhibited …
stress, and mechanisms have been considered to depend exclusively on its enzyme activity.
This study aimed to examine if the protein lacking catalytic activities could also display such
resistance against oxidative stress. Stable transfectants of rat wild type HO-1 cDNA (HO-1-
U937) and those of its H25A mutant gene (mHO-1-U937) were established using human
monoblastic lymphoma cell U937. HO-1-U937 and mHO-1-U937 used in the study exhibited …