Effects of busulfan on murine spermatogenesis: cytotoxicity, sterility, sperm abnormalities, and dominant lethal mutations
LR Bucci, ML Meistrich - Mutation Research/Fundamental and Molecular …, 1987 - Elsevier
The alkylating agent busulfan (Myleran) adversely affects spermatogenesis in mammals. We
treated male mice with single doses of busulfan in order to quantitate its cytotoxic action on
spermatogonial cells for comparison with effects of other chemotherapeutic agents, to
determine its long-term effects on fertility, and to assessits possible mutagenic action. Both
stem cell and differentiating spermatogonia were killed and, at doses above 13 mg/kg, stem
cell killing was more complete than that of differentiating spermatogonia. Azoospermia at 56 …
treated male mice with single doses of busulfan in order to quantitate its cytotoxic action on
spermatogonial cells for comparison with effects of other chemotherapeutic agents, to
determine its long-term effects on fertility, and to assessits possible mutagenic action. Both
stem cell and differentiating spermatogonia were killed and, at doses above 13 mg/kg, stem
cell killing was more complete than that of differentiating spermatogonia. Azoospermia at 56 …