Assessment of IgG antibodies against yellow fever virus after vaccination with 17D by different assays: neutralization test, haemagglutination inhibition test …

M Niedrig, M Lademann, P Emmerich… - Tropical Medicine & …, 1999 - Wiley Online Library
M Niedrig, M Lademann, P Emmerich, M Lafrenz
Tropical Medicine & International Health, 1999Wiley Online Library
We analysed serum samples of 209 subjects immunized with yellow fever vaccine 17D by
different assays: neutralization test, immunofluorescence assay, haemagglutination
inhibition test and ELISA, for presence of 17D‐specific antibodies. Serum samples were
taken from a few weeks up to 35 years after vaccination. The neutralization test had the
highest sensitivity. There was no correlation of results between the serological assays.
Considering NT titres> 1: 10 as indicating protection, we found that about 75% of subjects …
Summary
We analysed serum samples of 209 subjects immunized with yellow fever vaccine 17D by different assays: neutralization test, immunofluorescence assay, haemagglutination inhibition test and ELISA, for presence of 17D‐specific antibodies. Serum samples were taken from a few weeks up to 35 years after vaccination. The neutralization test had the highest sensitivity. There was no correlation of results between the serological assays. Considering NT titres > 1:10 as indicating protection, we found that about 75% of subjects remained immune even 10 years after vaccination, with a median NT titre of 1:40 in reactive sera.
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