[HTML][HTML] Expansion of human umbilical cord blood SCID-repopulating cells using chromatin-modifying agents
OBJECTIVE: We investigated whether the addition of two chromatin-modifying agents, 5-aza-
2′-deoxycytidine (5azaD) and trichostatin A (TSA), to cord blood (CB) CD34+ cells in
culture results in expansion of the numbers of severe combined immunodeficient (SCID)
repopulating cells (SRC). MATERIALS AND METHODS: Human CB CD34+ cells were
cultured with cytokines in the presence or absence of 5azaD/TSA. After 9 days of culture, the
fold expansion of CD34+ and CD34+ CD90+ cell numbers, colony-forming unit (CFU)-mix …
2′-deoxycytidine (5azaD) and trichostatin A (TSA), to cord blood (CB) CD34+ cells in
culture results in expansion of the numbers of severe combined immunodeficient (SCID)
repopulating cells (SRC). MATERIALS AND METHODS: Human CB CD34+ cells were
cultured with cytokines in the presence or absence of 5azaD/TSA. After 9 days of culture, the
fold expansion of CD34+ and CD34+ CD90+ cell numbers, colony-forming unit (CFU)-mix …