[HTML][HTML] Interactions between chemokines: regulation of fractalkine/CX3CL1 homeostasis by SDF/CXCL12 in cortical neurons
A Cook, R Hippensteel, S Shimizu, J Nicolai… - Journal of biological …, 2010 - ASBMB
The soluble form of the chemokine fractalkine/CX 3 CL1 regulates microglia activation in the
central nervous system (CNS), ultimately affecting neuronal survival. This study aims to
determine whether CXCL12, another chemokine constitutively expressed in the CNS
(known as stromal cell-derived factor 1; SDF-1), regulates cleavage of fractalkine from
neurons. To this end, ELISA was used to measure protein levels of soluble fractalkine in the
medium of rat neuronal cultures exposed to SDF-1. Gene arrays, quantitative RT-PCR, and …
central nervous system (CNS), ultimately affecting neuronal survival. This study aims to
determine whether CXCL12, another chemokine constitutively expressed in the CNS
(known as stromal cell-derived factor 1; SDF-1), regulates cleavage of fractalkine from
neurons. To this end, ELISA was used to measure protein levels of soluble fractalkine in the
medium of rat neuronal cultures exposed to SDF-1. Gene arrays, quantitative RT-PCR, and …