" Soluble" adenylyl cyclase (sAC) is a widely expressed source of cAMP in mammalian cells
that is evolutionarily, structurally, and biochemically distinct from the G protein-responsive
transmembrane adenylyl cyclases. In contrast to transmembrane adenylyl cyclases, sAC is
insensitive to heterotrimeric G protein regulation and forskolin stimulation and is uniquely
modulated by bicarbonate ions. Here we present the first report detailing kinetic analysis
and biochemical properties of purified recombinant sAC. We confirm that bicarbonate …