Cell-mediated cytotoxicity is a crucial mechanism involved in several fundamental
immunological processes such as protection against intracellular pathogens or termination
of an immune response. This phenomenon is classically evaluated by the 51Cr release
assay, which requires a radioactive isotope and does not permit the characterization of cells
involved in the cytotoxic reaction. We describe a new flow cytometry method, developed in
the context of CD95-mediated cell death, which allows the precise quantitation of cell …